BioToxin Assays
Acumen Detection’s patent-pending toxin assays deliver fluorescence based, near real-time, one-step detection. The assays also work on PCR platforms, eliminating the need for responders to carry a second instrument to the field to identify toxins. The toxin assays are run at a steady 37° C, and not thermal cycled. The assays are “open architecture”; can be run on virtually any qPCR platform. Positive and negative controls are provided.
Availability: DISCONTINUED
| Manufacturer |
| Acumen Detection |
| Product Name |
| BioToxin Assays |
| Detection Category |
| Biological |
| Detection Principle |
| Immunological; |
| Detection Method |
| Lateral Flow Hand Held Immunoassay; |
| Application |
| Forensics; |
| Product Model Number |
| Acu-Bot™, Acu-Ricin™ and Acu-PAPA™ , Acu-Shiga ™ |
| Equipment Type |
| Instrument |
| Product Synopsis |
| Acumen Detection’s patent-pending toxin assays deliver fluorescence based, near real-time, one-step detection. The assays also work on PCR platforms, eliminating the need for responders to carry a second instrument to the field to identify toxins. The toxin assays are run at a steady 37° C, and not thermal cycled. The assays are “open architecture”; can be run on virtually any qPCR platform. Positive and negative controls are provided. |
| Availability |
| DISCONTINUED |
| Technology Readiness Level (TRL) |
| 8 |
| User Feedback Sources |
| Department of Defense as part of JBTDS Phase 1, and JUPITR ATD, DHS
BioWatch. |
| Weight |
| <1 lb. |
| Power Requirements |
| None |
| Transportability |
| Handheld |
| Consumables |
| None |
| Solvents/Reagents |
| Assays are lyophilized (freeze dried) in reaction volumes |
| Expected Operational Life |
| One time use |
| Shelf Life |
| <5 years; 1 to 2 years at 4°C, 1 month to 2 years at 25°C, 10 to 16 weeks at 50°C. |
| Unit Cost |
| $14 to $21 per individual assay |
| Tested For Biological Agents |
| B. anthracis (Anthrax), F. tularensis (Tularemia), Y. Pestis (Plague), Brucella, C. burnetii (Q fever), pan-orthopox, Venezuelan equine encephalitis (VEE), Ricin, Botulism toxin, Staphlyococcal Enterotoxin B (SEB), Shiga toxin |
| Sample Introduction |
| Liquid; |
| Sensitivity/Detection Limits |
| ng; Anthrax, Tularemia, Plague, Brucella, Q fever 133 org/mL, SEB 50 ng/mL, Ricin 160 ng/mL, Botulinum 10 ng/mL, |
| Known Interferents/Inhibitors |
| Assays are formulated to be tolerant of
environmental contaminants (interferents) like dust, humic acid, salts, diesel exhaust, etc. As long as the sample is in an aqueous solution or suspension, our assays can accept it.
Extremely heavy inhibitor load in the samples resulting in false negative results.Non-specific proteases in the sample matrix creating false positives in the toxin assays. Negative control assays are provided to be run concurrent with our toxin assays. |
| False Positive Rates |
| No false positives were seen in vendor testing. |
| False Negative Rates |
| 1% |
| Total Run Time |
| 1 hour |
| Other Operational Parameters |
| 100 µL sample volume |
| Training Required |
| <1 hour training |
| Training Available |
| Yes |
| Manuals Available |
| Yes |
