QX200 Droplet Digital PCR System
The Bio-Rad QX200 ddPCR System combines water-oil emulsion droplet technology with microfluidics. The system consists of four instruments: a droplet generator, a plate sealer, a thermo cycler, and a droplet reader. The QX200 droplet generator, which can process eight samples at a time and requires samples to be manually pipetted into sample wells, partitions each sample into 20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. Each droplet serves to partition the reactions. Droplets are transferred to a 96-well PCR plate and PCR is performed to end point in a thermal cycler. The droplets stream single file through the reader for fluorescence analysis. Positive droplets, which contain at least one copy of the target DNR or RNA molecule, exhibit increased fluorescence compared to negative droplets. The fraction of PCR-positive droplets enables the target to be quantified according to the Poisson distribution.
Availability: Commercially Available
+1 800 424 6723
2000 Alfred Nobel Drive
Hercules, CA 94547
USA
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www.bio-rad.com
| Manufacturer |
| BioRad Laboratories, Inc. |
| Product Name |
| QX200 Droplet Digital PCR System |
| Detection Category |
| Biological |
| Detection Principle |
| Molecular; Molecular Spectroscopy; |
| Detection Method |
| Polymerase Chain Reaction; Fluorescence ; |
| Application |
| Detection; Laboratory Analysis; |
| Equipment Type |
| Instrument |
| Product Synopsis |
| The Bio-Rad QX200 ddPCR System combines water-oil emulsion droplet technology with microfluidics. The system consists of four instruments: a droplet generator, a plate sealer, a thermo cycler, and a droplet reader. The QX200 droplet generator, which can process eight samples at a time and requires samples to be manually pipetted into sample wells, partitions each sample into 20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. Each droplet serves to partition the reactions. Droplets are transferred to a 96-well PCR plate and PCR is performed to end point in a thermal cycler. The droplets stream single file through the reader for fluorescence analysis. Positive droplets, which contain at least one copy of the target DNR or RNA molecule, exhibit increased fluorescence compared to negative droplets. The fraction of PCR-positive droplets enables the target to be quantified according to the Poisson distribution. |
| Availability |
| Commercially Available |
| Technology Readiness Level (TRL) |
| 9 |
| User Feedback Sources |
| Indifferent/No user feedback |
| Supporting Documentation |
| Docs: BIORAD - QX200 Droplet Reader.pdf; BIORAD - QX200 Auto Droplet Generator.pdf; |
| Dimensions |
| Droplet Generator: 11 x 14 x 5 in (28 x 36 x 13 cm), Droplet reader: 26 x 20 x 11 in (66 x 52 x 29 cm) |
| Weight |
| >50 Lbs.; Droplet Generator: 10 Lbs. (4.5 kg), Droplet reader: 56.6 Lbs. (26 kg) |
| Power Requirements |
| AC/DC Line Power; 100 to 240 V AC, 60W |
| Transportability |
| Person-Portable |
| Operating Conditions |
| 18 to 30°C (64 to 86°F), <85%RH, non-condensing |
| Solvents/Reagents |
| Yes |
| Suggested Routine Maintenance |
| Removal and disposal of droplet waste |
| Available Accessories |
| Sample prep kits: pipettes, master mix, buffer, primers, PCR plates, etc. |
| Data Analysis Support Equipment |
| QuantaSoft Analysis Pro software suitable with Windows 7, 10 |
| Communications Interface |
| USB 2.0 |
| Expected Operational Life |
| Indefinite |
| Shelf Life |
| >5 years |
| Unit Cost |
| $100,000.00 |
| Other Biological Targets |
| DNA, RNA, SARS-CoV-2 (COVID-19) |
| Adaptability |
| Yes |
| Sample Introduction |
| Liquid |
| Known Interferents/Inhibitors |
| Sample must be cleaned and free of interferents. |
| Start Up Time (From Cold Start To Sample Ready) |
| 30 minutes |
| Response Time (Sample Application To Output) |
| >15 minutes; 1 hour |
| Alarm Capability |
| No |
| Software Control |
| On-board, PC |
| Training Required |
| <1 day training |
| Training Available |
| Yes |
| Manuals Available |
| Yes |
| Data File Type Format |
| qlp, ddpcr, ddplt |
