FACSCount
Flow cytometry technology adapted for use with bacteria, yeast, mold, and others. Automated sample prep system, a fluidic system, a sample handling system, and an optical bench with data processing electronics, and software. The FACSMicroCount prepares samples by labeling with a nucleic acid dye and the BRAG3 labeling compound. The sample passes through the laser beam where individual microbes absorb, scatter, or transmit the laser light. Labeled bacteria will scatter the laser light as well as emit laser-induced fluorescence. A scatter and fluorescence intensity for each individual microbe is displayed for analysis. Licensed from Advanced Analytical Technologies, Inc which previously sold the instrument under the Micro Pro and RBD3000 names.
Availability: Commercially Available
| Manufacturer |
| BD Biosciences |
| Product Name |
| FACSCount |
| Detection Category |
| Biological |
| Detection Principle |
| Flow Cytometry; Molecular Spectroscopy; |
| Detection Method |
| Flow Cytometry; Fluorescence; |
| Application |
| Detection; Laboratory Analysis; |
| Equipment Type |
| Instrument |
| Product Synopsis |
| Flow cytometry technology adapted for use with bacteria, yeast, mold, and others. Automated sample prep system, a fluidic system, a sample handling system, and an optical bench with data processing electronics, and software. The FACSMicroCount prepares samples by labeling with a nucleic acid dye and the BRAG3 labeling compound. The sample passes through the laser beam where individual microbes absorb, scatter, or transmit the laser light. Labeled bacteria will scatter the laser light as well as emit laser-induced fluorescence. A scatter and fluorescence intensity for each individual microbe is displayed for analysis. Licensed from Advanced Analytical Technologies, Inc which previously sold the instrument under the Micro Pro and RBD3000 names. |
| Availability |
| Commercially Available |
| Technology Readiness Level (TRL) |
| 9 |
| User Feedback Sources |
| Indifferent/No user feedback |
| Dimensions |
| 22.4 x 35.4 x 25.2 in (57 x 90 x 64 cm) |
| Weight |
| >50 lbs.; 230 lbs (104 kg) |
| Power Requirements |
| AC/DC Line Power; 100 to 120 V AC |
| Transportability |
| Benchtop/Fixed |
| Ruggedness |
| None; Fragile |
| Operating Conditions |
| 15 to 40°C (59 to 105°F) |
| Consumables |
| >$1,000/year |
| Suggested Routine Maintenance |
| Laser alignment in the flow cell |
| Available Accessories |
| Pipette, tubing, vortex mixer. Computer workstation bundle. |
| Communications Interface |
| USB connectivity to PC |
| Shelf Life |
| >5 years; Depends on the reagents used in the instrument—any unique storage condition of reagents (refrigeration), etc. |
| Unit Cost |
| $116,000.00 |
| Other Biological Targets |
| Whole cells—bacteria, yeast, mold |
| Adaptability |
| Yes, user can add new targets to detector with support—dye-conjugated antibodies can be used against analyte-of-interest for visualization |
| Sample Introduction |
| Liquid |
| Sensitivity/Detection Limits |
| cfu/mL; 10^1 cfu/mL; 10^6 cfu/mL |
| Known Interferents/Inhibitors |
| Sample must be free of particulates larger than the instrument's measuring capability of > 20 micrometers in diameter. |
| Start Up Time (From Cold Start To Sample Ready) |
| 20 to 30 minutes (includes reagent preparation, instrument preparation and running controls) |
| Response Time (Sample Application To Output) |
| >15 minutes; 5 min to 24 hours, depending on application |
| Alarm Capability |
| Visual |
| Other Operational Parameters |
| Recovery Time: Dependent on the application. Purified water, swab testing, and pure culture enumeration will be 15 to 18 minutes per sample. Product testing on semi-viscous or viscous products will be 8 minutes per sample after 24 hr enrichment. Antibody testing is application specific. |
| Training Required |
| >1 week training |
| Warranties |
| 1 year |
| IATA Shipping Restrictions |
| No |
