LightCycler Real-Time PCR System

The LightCycler's small reaction vessels allow for efficient temperature ramping, with cycling rates of less than 20 seconds/cycle. The LightCycler Nano can perform duplex assays on up to 32 samples. The LightCycler is available in 96, 480 and 1536 sample configurations each of which can perform 4 dye multiplexing.

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Availability: Commercially Available

+1 800 262 4911
9115 Hague Road, P.O. Box 50414
Indianapolis, Indiana 46250
USA
https://lifescience.roche.com/
Manufacturer
Roche Diagnostics Corporation
Product Name
LightCycler Real-Time PCR System
Detection Category
Biological
Detection Principle
Molecular; Molecular Spectroscopy;
Detection Method
Polymerase Chain Reaction; Fluorescence;
Application
Laboratory Analysis;Diagnostics;
Equipment Type
Instrument
Product Synopsis
The LightCycler's small reaction vessels allow for efficient temperature ramping, with cycling rates of less than 20 seconds/cycle. The LightCycler Nano can perform duplex assays on up to 32 samples. The LightCycler is available in 96, 480 and 1536 sample configurations each of which can perform 4 dye multiplexing.
Availability
Commercially Available
Technology Readiness Level (TRL)
9
User Feedback Sources
MRIGlobal hands-on experience
Dimensions
17.7 x 11.8 x 15.7 in (45 x 30 x 40 cm)
Weight
<50 Lbs.; 48.5 Lbs. (22 kg)
Power Requirements
AC/DC Line Power; 115 V (60 Hz); 230 V (50 Hz); Reader, 400mA; Processor, SP 1.9 A
Transportability
Benchtop/Fixed; Small Footprint
Operating Conditions
18 to 30°C (64 to 86°F)
Solvents/Reagents
Yes
Calibration Schedule
Automatic
Suggested Routine Maintenance
Bulb replacement for fluorophore excitation
Available Accessories
Sample preparation materials
Communications Interface
Serial interface connection to computer
Expected Operational Life
indefinite
Shelf Life
>5 years
Unit Cost
$28,900.00
Maintenance Cost
$2 per sample
Tested For Biological Agents
Ebola, RNA/DNA
Other Biological Targets
HSV, MRSA, strep A/B
Adaptability
Yes; user can add new targets in the field
Sample Introduction
Liquid
Start Up Time (From Cold Start To Sample Ready)
2 to 3 minutes
Response Time (Sample Application To Output)
>15 minutes; 20 to 30 minutes. This does not include time required for nucleic acid extraction and purification.
Alarm Capability
Visual
Software Control
PC
Training Required
>1 week training
Training Available
Yes
Manuals Available
Yes
Data File Type Format
ATF; COF; IXO; SAM
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