GeneXpert

The GeneXpert® is a cartridge based sample preparation and real-time PCR analysis system. The GeneXpert® uses disposable cartridges to move a sample through the preparation and analysis steps. DNA is extracted from the sample in a cell lysing step (sonication), mixed with the appropriate reagents, then analyzed by PCR. Multiple models are available with 1, 2, 4, 16, 48 or 80 module configurations for increased sample throughput.

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Availability: Commercially Available

Paul Boschee
Director of Corporate Accounts
paul.boschee@cepheid.com
+1 408 400 4809
904 Caribbean Drive
Sunnyvale, CA 94089
USA
www.cepheid.com
Manufacturer
Cepheid
Product Name
GeneXpert
Detection Category
Biological
Detection Principle
Molecular; Molecular Spectroscopy;
Detection Method
Polymerase Chain Reaction; Fluorescence ;
Application
Laboratory Analysis;
Equipment Type
Instrument
Product Synopsis
The GeneXpert® is a cartridge based sample preparation and real-time PCR analysis system. The GeneXpert® uses disposable cartridges to move a sample through the preparation and analysis steps. DNA is extracted from the sample in a cell lysing step (sonication), mixed with the appropriate reagents, then analyzed by PCR. Multiple models are available with 1, 2, 4, 16, 48 or 80 module configurations for increased sample throughput.
Availability
Commercially Available
Technology Readiness Level (TRL)
9
User Feedback Sources
Indifferent/No user feedback
Dimensions
GX-I: 4.00 x 12.00 x 11.70 in (102 x 305 x 298 mm) GX-II: 6.35 x 12.00 x 11.70 in (162 x 305 x 298 mm) GX-IV: 11.00 x 12.00 x 11.70 (280 x 305 x 298 mm) GX-XVI: 22.75 x 25.80 x 13.25 (578 x 656 x 337 mm)
Weight
<30 lbs.; GXI: 18 lbs. (8.2 kg)\ for 1 module instrument GXIV: 26 lbs. (11.8 kg) for 4 module instrument
Power Requirements
AC/DC Line Power; 100 to 240 V AC, 50 to 60 Hz, 1.9 to 0.95 A for GXIV and 1.5 to 7.5A for GXI
Transportability
Benchtop/Fixed; Small Footprint
Operating Conditions
15 to 30°C (59 to 86°F)
Calibration Schedule
Calibration after 2,000 runs
Suggested Routine Maintenance
Calibration after 2,000 runs
Data Analysis Support Equipment
PC
Communications Interface
USB
Shelf Life
>5 years
Unit Cost
$24,000 to $79,200
Maintenance Cost
$42 per cartridge
Tested For Biological Agents
B. anthracis (Anthrax), Ebola, Nucleic acids
Other Biological Targets
MRSA, nurovirus, Influenza, Entero virus, Strep A SARS-CoV-2 (COVID-19)
Sample Introduction
Liquid; Solid
Known Interferents/Inhibitors
DNA extraction performed in cartridge usually removes PCR interfering substances but test could be susceptible to failure due to clogging of pre-filter by particulates. In an independent laboratory study, the failure rate was as high as 1 in 3 with approximately 200 highly particulate samples.
Start Up Time (From Cold Start To Sample Ready)
< 1 minute
Response Time (Sample Application To Output)
>15 minutes; > 25 minutes
Alarm Capability
Yes
Software Control
PC
Training Required
<1 week training
Training Available
Although expensive to use at $50/run, the GeneXpert requires very little training to operate, unlike most real-time PCR systems. The sample is not pre-filtered, so dirty samples can cause cartridge failure. Samples containing PCR inhibitors may also impede performance. Due to cartridge variability, the GeneXpert is not a quantitative system.
Warranties
1 year. A second year extended warranty is available for purchase for $10,000
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