Scanning electron
microscopy (SEM) is an imaging technique that is used to produce high
resolution surface images of a sample. In
SEM, the sample is bombarded with electrons from an electron gun. The electron
beam is raster across the sample and secondary electrons and back scatter
electrons can be observed to form the final image. SEM can provide a resolution
of better than one nanometer. The technique
can be used to image any solid sample that fits onto the specimen holder. It has been more recently employed to image
biological samples as well. SEM instruments can
be coupled with detector systems such as energy dispersive x-ray spectroscopy
(EDS or EDX). This provides elemental
information about the composition of the sample surface. SEM instruments
range in size from benchtop instruments to large free standing models. The technology is mature.