Polarized light
microscopy is a non-destructive technique which uses plane-polarized visible
light to interrogate samples. The polarization of the light source leads to
enhanced image contrast in materials that have directionally-dependent
refractive indices (birefringent). In
PLM, light passes through a polarizer prior to interacting with the specimen
and through an analyzer before reaching the observation optics (either
eyepieces or camera). The morphological character of a sample may be obtained
by PLM as well as information about homogeneity, crystal structure, origin
(natural versus synthetic). Additionally, with the addition of components such
as hot stages and index matching oils, the refractive index of materials may
also be determined by PLM. While most
broadly used in the fields of geology and materials science, PLM can also be
applied to forensic, pharmaceutical, and biological samples as well. The
combination of information obtained from PLM alone can often be used to
identify unknown materials. Some
microscopes can be coupled to cameras for image preservation. Microscopes are typically employed as benchtop instruments. It is an extremely mature analytical
technique and instruments are widely available from a large number of
manufacturers. Microscope prices vary
widely depending on the quality and complexity of the lens optics and
accessories such as camera and hot stages.