Home > Biological Detection > Technology > Molecular > Polymerase Chain Reaction (PCR)

Real-time Polymerase Chain Reaction (PCR) amplification is generally regarded as the most sensitive and specific means of detecting potential bio warfare pathogens. Results can usually be obtained in about an hour, including a separate sample preparation step to manually extract DNA signatures prior to PCR analysis. Well-characterized COTS DNA extraction kits are available for rapid extraction (20 to 30 minutes) of target DNA from a variety of complex liquid and solid matrices including food, water, and tissue. Samples are prepared by the end user and the instrument performs PCR amplification of the target sequences. Thermal cycling produces additional copies of target sequences, and the fluorescent levels in the reaction change as additional copies are produced. During thermal cycling, the instrument detects copies of the target sequences by measuring changes in fluorescence.

It should be noted that nucleic acid-based detection devices do not directly detect non-nucleic acid targets such as toxins, as inherent in the technology. However, toxins may be indirectly detected via the presence of residual DNA fragments of the organism used to produce the toxin. Residual DNA fragments from the organism used to produce a toxin are typically present at low levels even in the purest of toxin preparations, and fragments are most likely present in higher concentrations in crude preparations.

Manufacturer
Agilent Technologies (1)
Biomeme, Inc. (1)
bioMérieux, Inc. (3)
Bioneer (1)
BioRad (2)
Cepheid (2)
GeneReach USA (1)
Life Technologies (1)
Micronics (1)
Nanosphere, Inc. (1)
Pall Corporation (3)
Pathogenica (1)
PositiveID/MicroFluidic Systems (3)
Roche Diagnostics Corporation (1)
Tetracore, Inc. (1)
Thermo Fisher Scientific, Inc. (1)
Veredus Laboratories (1)
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Transportability
Pocket Size (2)
Handheld (7)
Person-Portable (9)
Mounted (1)
Benchtop/Fixed (6)
Availability
Commercially Available (19)
Discontinued (2)
In Development (4)
Unit Cost
$5,000 - $10,000 (1)
$20,000 - $50,000 (8)
N/A (16)
Sample Introduction
Solid (5)
Liquid (19)
Vapor/Gas (1)
Sensitivity / Detection Limit
ppq (1)
cfu/mL (1)
ng (3)
copies/mL (1)
Ci (6)
Other (5)
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7500 Fast Real-Time PCR System

7500 Fast Real-Time PCR System

Life Technologies

The Applied Biosystems 7500 Fast Real-Time PCR System is a real-time PCR system which enables high-...

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CFX Touch Series

CFX Touch Series

BioRad

The Bio-Rad CFX Touch is a real-time PCR detection system for use with SYBR dyes or Taqman probes. T...

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DragonFly

DragonFly

PositiveID/MicroFluidic Systems

The MicroFluidic System branch of PositiveID has developed a series of cartridges for biological sam...

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ExiProgen

ExiProgen

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FilmArray

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The FilmArray integrates sample preparation, amplification, detection, and analysis into one system ...

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Firefly Dx

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The Firefly Dx system combines a single use sample prep and reagent PCR cartridge with a bluetooth e...

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GeneDisc® Plates – Food Pathogen Detection

GeneDisc® Plates – Food Pathogen Detection

Pall Corporation

The product offers a complete ‘sample to result’ method. Differentiating between the most pathogeni...

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GeneDisc® Plates – Legionella Detection

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Pall Corporation

Process control, monitoring and traceability are becoming increasingly important within the healthca...

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GeneDisc® Rapid Microbiology System

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The system consists of three key components - the GeneDisc DNA Extractor, GeneDisc plate and GeneDi...

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GeneXpert

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The GeneXpert® is a cartridge based sample preparation and real-time PCR analysis system. The GeneXp...

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HAI BioDetection System

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Pathogenica’s real-time BioSurveillance and BioDetection system detects high resolution fingerprint ...

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LightCycler Real-Time PCR System

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The LightCycler's small reaction vessels allow for quick and efficient temperature ramping. Cycling ...

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M-BAND

M-BAND

PositiveID/MicroFluidic Systems

The Microfluidics-based Bioagent Networked Detector (M-BAND) developed by MicroFluidic Systems (MFS)...

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Mx3000P Realtime PCR

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Agilent Technologies

The device is a real-time PCR instrument capable of four dye multiplexing. The Mx3000P's single sour...

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PanNAT System

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Micronics’ PanNAT molecular diagnostic system is a convenient, point of care instrument capable of p...

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POCKIT™ Nucleic Acid Analyzer

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GeneReach USA

The POCKIT™ is a PCR-based platform that can measure eight samples in parallel. After several sample...

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