Home > Biological Detection > Technology > Molecular > Polymerase Chain Reaction (PCR)

Real-time Polymerase Chain Reaction (PCR) amplification is generally regarded as the most sensitive and specific means of detecting potential bio warfare pathogens. Results can usually be obtained in about an hour, including a separate sample preparation step to manually extract DNA signatures prior to PCR analysis. Well-characterized COTS DNA extraction kits are available for rapid extraction (20 to 30 minutes) of target DNA from a variety of complex liquid and solid matrices including food, water, and tissue. Samples are prepared by the end user and the instrument performs PCR amplification of the target sequences. Thermal cycling produces additional copies of target sequences, and the fluorescent levels in the reaction change as additional copies are produced. During thermal cycling, the instrument detects copies of the target sequences by measuring changes in fluorescence.

It should be noted that nucleic acid-based detection devices do not directly detect non-nucleic acid targets such as toxins, as inherent in the technology. However, toxins may be indirectly detected via the presence of residual DNA fragments of the organism used to produce the toxin. Residual DNA fragments from the organism used to produce a toxin are typically present at low levels even in the purest of toxin preparations, and fragments are most likely present in higher concentrations in crude preparations.

Manufacturer
Agilent Technologies (1)
Biomeme, Inc. (1)
bioMérieux, Inc. (3)
Bioneer (1)
BioRad (2)
Cepheid (2)
GenArraytion, Inc. (92)
GeneReach USA (1)
Life Technologies (1)
Micronics (1)
Nanosphere, Inc. (1)
Pall Corporation (3)
Pathogenica (1)
PositiveID/MicroFluidic Systems (3)
Roche Diagnostics Corporation (1)
Tetracore, Inc. (1)
Thermo Fisher Scientific, Inc. (1)
Veredus Laboratories (1)
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Transportability
Pocket Size (31)
Handheld (70)
Person-Portable (9)
Mounted (1)
Benchtop/Fixed (6)
Availability
Commercially Available (111)
Discontinued (3)
In Development (3)
Unit Cost
< $100 (85)
$100 - $500 (7)
$5,000 - $10,000 (1)
$20,000 - $50,000 (8)
N/A (16)
Sample Introduction
Solid (5)
Liquid (111)
Vapor/Gas (1)
Sensitivity / Detection Limit
ppq (1)
cfu/mL (1)
ng (3)
copies/mL (1)
Ci (6)
Other (5)
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7500 Fast Real-Time PCR System

7500 Fast Real-Time PCR System

Life Technologies

The Applied Biosystems 7500 Fast Real-Time PCR System is a real-time PCR system which enables high-...

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Babesia microti RT-PCR Assay

Babesia microti RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Babesia microti (ROX). GenArraytion's Bioassays are read...

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Borrelia afzelli RT-PCR Assay

Borrelia afzelli RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Borrelia afzelli (FAM). GenArraytion's Bioassays are rea...

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Borrelia garinii RT-PCR Assay

Borrelia garinii RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Borrelia garinii (FAM). GenArraytion's Bioassays are rea...

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Borrelia miyamotoi RT-PCR Assay

Borrelia miyamotoi RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Borrelia miyamoto (FAM). GenArraytion's Bioassays are re...

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CFX Touch Series

CFX Touch Series

BioRad

The Bio-Rad CFX Touch is a real-time PCR detection system for use with SYBR dyes or Taqman probes. T...

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Coxiella burnetti RT-PCR Assay

Coxiella burnetti RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination Coxiella burnetti (Cy5). GenArraytion's Bioassays are readil...

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DragonFly

DragonFly

PositiveID/MicroFluidic Systems

The MicroFluidic System branch of PositiveID has developed a series of cartridges for biological sam...

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Ehrlichia canis RT-PCR Assay

Ehrlichia canis RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Ehrlichia canis (FAM). GenArraytion's Bioassays are read...

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Ehrlichia chaffeensis RT-PCR Assay

Ehrlichia chaffeensis RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Ehrlichia chaffeensis (FAM). GenArraytion's Bioassays ar...

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ExiProgen

ExiProgen

Bioneer

Bioneer's ExiProgen™ is a fully automated system for various scientific purposes in synthetic biolog...

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FilmArray

FilmArray

bioMérieux, Inc.

The FilmArray integrates sample preparation, amplification, detection, and analysis into one system ...

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Firefly Dx

Firefly Dx

PositiveID/MicroFluidic Systems

The Firefly Dx system combines a single use sample prep and reagent PCR cartridge with a bluetooth e...

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Francisella tularensis RT-PCR Assay

Francisella tularensis RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Francisella tularensis (FAM). GenArraytion's Bioassays a...

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GeneDisc® Plates – Food Pathogen Detection

GeneDisc® Plates – Food Pathogen Detection

Pall Corporation

The product offers a complete ‘sample to result’ method. Differentiating between the most pathogeni...

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GeneDisc® Plates – Legionella Detection

GeneDisc® Plates – Legionella Detection

Pall Corporation

Process control, monitoring and traceability are becoming increasingly important within the healthca...

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