Molecular assays target nucleic acid (DNA or RNA) sequences to demonstrate the presence of a particular target(s) within a sample, in contrast to immunoassays, which target proteins. Most commercial molecular assays are based on PCR (the Polymerase Chain Reaction), an enzymatic amplification method that amplifies the target sequence to improve the limit of detection and time to detect. The PCR process involves forward and reverse primers, which define the target region.

Real-Time PCR assays include a third nucleic acid sequence, termed a probe, which binds a region between the amplification primers and is tagged with a fluorescent molecule and a fluorescence quencher. Thermal cycling is key to the PCR process and involves repeated rounds of heating and cooling. The reaction tube is heated to dissociate the double-stranded DNA template. As the reaction tube is cooled, the primers and probe bind to the single stranded DNA. The PCR enzyme synthesizes a new second DNA strand based on the primer “start button” and removes any DNA in it’s path as it proceeds down the strand, thereby dissociating the probe and releasing the fluorescent molecule into solution where it is “seen” by the PCR instrument’s detection element. Changes in the fluorescence properties of the reaction tube indicate that the particular target sequence is present in the sample and is being amplified. 

Direct hybridization assays employee coded bead sets, in which each bead set is functionalized with a capture oligo specific for a nucleic acid sequence (referred to as the capture oligonucleotide) in the target that binds the amplicon product of the PCR reaction, thereby demonstrating the presence of the target nucleic acid sequence.

An advantage of real-time PCR is a relatively short time to detect, typically in 15-120 minutes (instrument dependent). An advantage of bead-based assays is that a sample can be interrogated for more targets in a single tube; up to 50 vs. 4 to 6 targets for real-time PCR, which is constrained by overlap in the fluorescence spectrum of available probes.

Manufacturer
GenArraytion, Inc. (29)
Transportability
Pocket Size (29)
Availability
Commercially Available (29)
Unit Cost
< $100 (22)
$100 - $500 (7)
Sample Introduction
Liquid (29)
Sensitivity / Detection Limit
Grid ViewList View | Compare Products | Products per page:416100All
Babesia microti RT-PCR Assay

Babesia microti RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Babesia microti (ROX). GenArraytion's Bioassays are read...

Compare Compare Now  
Borrelia afzelli RT-PCR Assay

Borrelia afzelli RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Borrelia afzelli (FAM). GenArraytion's Bioassays are rea...

Compare Compare Now  
Borrelia garinii RT-PCR Assay

Borrelia garinii RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Borrelia garinii (FAM). GenArraytion's Bioassays are rea...

Compare Compare Now  
Borrelia miyamotoi RT-PCR Assay

Borrelia miyamotoi RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Borrelia miyamoto (FAM). GenArraytion's Bioassays are re...

Compare Compare Now  
Coxiella burnetti RT-PCR Assay

Coxiella burnetti RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination Coxiella burnetti (Cy5). GenArraytion's Bioassays are readil...

Compare Compare Now  
Ehrlichia canis RT-PCR Assay

Ehrlichia canis RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Ehrlichia canis (FAM). GenArraytion's Bioassays are read...

Compare Compare Now  
Ehrlichia chaffeensis RT-PCR Assay

Ehrlichia chaffeensis RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Ehrlichia chaffeensis (FAM). GenArraytion's Bioassays ar...

Compare Compare Now  
Francisella tularensis RT-PCR Assay

Francisella tularensis RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Francisella tularensis (FAM). GenArraytion's Bioassays a...

Compare Compare Now  
Japanese encephalitis virus (JEV) RT-PCR Assay

Japanese encephalitis virus (JEV) RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Japanese encephalitis virus (FAM). GenArraytion's Bioass...

Compare Compare Now  
Mayaro Virus RT-PCR Assay

Mayaro Virus RT-PCR Assay

GenArraytion, Inc.

Real-time PCR primer/probe combination for Mayaro virus (FAM). GenArraytion's Bioassays are readily...

Compare Compare Now  
MultiFLEX Aedes aegypti RT-PCR Assay

MultiFLEX Aedes aegypti RT-PCR Assay

GenArraytion, Inc.

Real-time multiplexed PCR primer/probe combination for Zika virus (VIC), Chikungunya virus (Cy5), De...

Compare Compare Now  
MultiFLEX Alpha Virus EEE, WEE, VEE RT-PCR Assay

MultiFLEX Alpha Virus EEE, WEE, VEE RT-PCR Assay

GenArraytion, Inc.

Real-time multiplexed PCR primer/probe combination for Eastern Equine Encephalitis Virus (FAM), West...

Compare Compare Now  
MultiFLEX Bacilllus anthracis Chromosome, pXO1, pXO2 RT-PCR Assay

MultiFLEX Bacilllus anthracis Chromosome, pXO1, pXO2 RT-PCR Assay

GenArraytion, Inc.

Real-time multiplexed PCR primer/probe combination for Bacillus anthracis detection. Probe for BA Ch...

Compare Compare Now  
MultiFLEX Borrelia burgdorferi and Anaplasma phagocytophilum RT-PCR assay

MultiFLEX Borrelia burgdorferi and Anaplasma phagocytophilum RT-PCR assay

GenArraytion, Inc.

Real-time multiplexed PCR primer/probe combination for Borrelia burgdorferi (Cy5), Anaplasma phagocy...

Compare Compare Now  
MultiFLEX Burkholderia mallei and Burkholderia pseudomallei RT-PCR Assay

MultiFLEX Burkholderia mallei and Burkholderia pseudomallei RT-PCR Assay

GenArraytion, Inc.

Real-time multiplexed PCR primer/probe combination for Burkholderia mallei (FAM) and Burkholderia ps...

Compare Compare Now  
MultiFLEX Clostridium difficile chromosome, Toxin A and Toxin B RT- PCR Assay

MultiFLEX Clostridium difficile chromosome, Toxin A and Toxin B RT- PCR Assay

GenArraytion, Inc.

Real-time multiplexed PCR primer/probe combination for Clostridium difficile chromosome (VIC), C. di...

Compare Compare Now